Fig. 3

High-throughput strategy for isolation of CO₂/HCO₃⁻ response mutants. Schematic of the screening strategy with 96-well culture plates. The leaves (red arrows) of 3-week-old AEQ-transgenic Arabidopsis were placed in a 96-well culture plate and 100 µL of incubation buffer was added to each well. Plates were incubated in the dark at 25 °C for 4 to 6 h. The wells were automatically injected with 100 µL of 2 mM KHCO₃ (to give a final concentration of 1 mM), and AEQ luminescence was recorded for each well