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Table 2 Primer and probes of endogenous reference genes used in this study

From: Development of a universal endogenous qPCR control for eukaryotic DNA samples

Target gene

Target species

Primer name

5′–3′ sequence

References

28S

Eukaryotes

UNI28S-fwd

CTACTATCTAGCGAAACC

This work

UNI28S-rev

AYTAGAGTCAAGCTCAAC

UNI28S-P

HEX-AAA + G+A + AG + A+C + C+C + T-DAB

cpLeu

Malus × domestica

qMD_cpLeu-fwd

CCTTCATCCTTTCTGAAGTTTCG

[24]

qMD_cpLeu-rev

AACAAATGGAGTTGGCTGCAT

qMD_cpLeu

HEX-TGGAAGGATTCCTTTACTAAC-TAMRA

aco

Malus × domestica

Md-ACO-F

CCAGAATGTCGATAGCCTCGTT

[25]

Md-ACO-R

GGTGCTGGGCTGATGAATG

Md-ACO

HEX-TACAACCCAGGCAACG

  1. A +prior to the nucleotide code indicates that the following nucleotide is an LNA