Development of a universal endogenous qPCR control for eukaryotic DNA samples

Mittelberger, Cecilia; Obkircher, Lisa; Oberkofler, Vicky; Ianeselli, Alan; Kerschbamer, Christine; Gallmetzer, Andreas; Reyes-Dominguez, Yazmid; Letschka, Thomas; Janik, Katrin

doi:10.1186/s13007-020-00597-2

Plant Methods

Table 1 Amplification results of eukaryotic species

From: Development of a universal endogenous qPCR control for eukaryotic DNA samples

Taxon	Species	Tested tissue	Mean C_q	Stdv C_q	28 S copies reaction⁻¹
Arthropod	Anaceratagallia ribauti	Whole insect	16.40	0.17	2,605,892
Arthropod	Aphrophora alni	Whole insect	8.65	0.72	561,670,136
Arthropod	Asymmetrasca decedens	Whole insect	16.85	0.27	1,916,323
Arthropod	Cacopsylla melanoneura	whole insect	16.52	0.02	14,145,566
Arthropod	Cacopsylla picta	Whole insect	16.24	0.07	17,121,902
Arthropod	Cicadula quadrinotata	Whole insect	14.51	0.08	9,706,053
Arthropod	Cixius nervosus	Whole insect	10.22	0.72	189,127,348
Arthropod	Dicranotropis hamata	Whole insect	13.84	0.19	15,409,055
Arthropod	Edwardsiana rosae	Whole insect	15.18	0.04	6,085,580
Arthropod	Emelyanoviana mollicula	Whole insect	16.18	0.14	3,042,300
Arthropod	Empoasca vitis	Whole insect	19.31	0.16	348,147
Arthropod	Eriosoma lanigerum	Whole insect	15.91	0.18	119,623,912
Arthropod	Ixodida^a	Whole insect	21.54	0.65	133,850
Arthropod	Laodelphax striatella	Whole insect	12.81	0.23	31,543,702
Arthropod	Macrosteles quadripunctulatus	Whole insect	20.38	0.12	165,800
Arthropod	Macrosteles cristatus	Whole insect	16.53	0.16	2,381,296
Arthropod	Macrosteles laevis	Whole insect	16.02	0.11	3,407,075
Arthropod	Macrosteles ossiannilssoni	Whole insect	15.15	0.06	6,213,481
Arthropod	Macrosteles sexnotatus	Whole insect	17.33	0.07	1,373,854
Arthropod	Psammotettix alienus	Whole insect	16.20	0.09	3,007,348
Arthropod	Psammotettix confinis	Whole insect	15.06	0.07	6,598,274
Arthropod	Stictocephala bisonia	Whole insect	9.42	0.90	330,858,020
Arthropod	Zygina flammigera	Whole insect	16.78	0.17	2,011,619
Arthropod	Zyginidia pullula	Whole insect	15.88	0.38	3,745,691
Fish	Salmo salar^a	Meat	20.69	0.11	240,091
Fungi	Boletus edulis^a	Stem part	15.42	0.28	9,270,902
Fungi	Saccharomyces cerevisiae^a	Colony	17.97	0.25	1,584,262
Mammal	Bos primigenius taurus^a	Meat	25.08	0.31	11,466
Mammal	Capreolus capreolus^a	Meat	22.57	0.57	65,416
Mammal	Equus ferus caballus^a	Hair	22.95	0.39	50,274
Mammal	Homo sapiens^a	Blood	26.57	0.93	4,103
Mammal	Mus musculus^a	Blood	24.09	0.23	22,820
Mammal	Ovis gmelini aries^a	Meat	22.19	0.36	84,923
Mammal	Sus scrofa^a	Meat	23.66	1.05	30,811
Plant	Ginkgo biloba^a	Leaf	19.89	0.23	418,889
Plant	Lycopersicon esculentum^a	Leaf	15.40	0.05	8,605,181
Plant	Malus × domestica^a	Root	20.73	1.69	233,529
Plant	Nicotiana occidentalis^a	Leaf	16.95	0.15	2,989,473
Plant	Olea europaea^a	Leaf	20.28	1.18	319,704
Plant	Pinus cembra^a	Leaf	17.68	0.31	1,932,346
Plant	Prunus armeniaca^a	Leaf	16.25	0.25	5,216,449
Plant	Pyrus communis^a	Leaf	20.72	0.53	235,152
Plant	Vitis vinifera^a	Leaf	18.04	0.21	1,509,260

Amplification results of the 28S rDNA amplicon in 43 different eukaryotic species with threshold cycle values (C_q) and quantification results based on the tenfold standard dilution series of pJet1.2-28S
^aTemplate DNA was diluted to 5 ng/µl

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ISSN: 1746-4811

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