Skip to main content

Table 1 Amplification results of eukaryotic species

From: Development of a universal endogenous qPCR control for eukaryotic DNA samples

TaxonSpeciesTested tissueMean CqStdv Cq28 S copies reaction−1
ArthropodAnaceratagallia ribautiWhole insect16.400.172,605,892
ArthropodAphrophora alniWhole insect8.650.72561,670,136
ArthropodAsymmetrasca decedensWhole insect16.850.271,916,323
ArthropodCacopsylla melanoneurawhole insect16.520.0214,145,566
ArthropodCacopsylla pictaWhole insect16.240.0717,121,902
ArthropodCicadula quadrinotataWhole insect14.510.089,706,053
ArthropodCixius nervosusWhole insect10.220.72189,127,348
ArthropodDicranotropis hamataWhole insect13.840.1915,409,055
ArthropodEdwardsiana rosaeWhole insect15.180.046,085,580
ArthropodEmelyanoviana molliculaWhole insect16.180.143,042,300
ArthropodEmpoasca vitisWhole insect19.310.16348,147
ArthropodEriosoma lanigerumWhole insect15.910.18119,623,912
ArthropodIxodidaaWhole insect21.540.65133,850
ArthropodLaodelphax striatellaWhole insect12.810.2331,543,702
ArthropodMacrosteles quadripunctulatusWhole insect20.380.12165,800
ArthropodMacrosteles cristatusWhole insect16.530.162,381,296
ArthropodMacrosteles laevisWhole insect16.020.113,407,075
ArthropodMacrosteles ossiannilssoniWhole insect15.150.066,213,481
ArthropodMacrosteles sexnotatusWhole insect17.330.071,373,854
ArthropodPsammotettix alienusWhole insect16.200.093,007,348
ArthropodPsammotettix confinisWhole insect15.060.076,598,274
ArthropodStictocephala bisoniaWhole insect9.420.90330,858,020
ArthropodZygina flammigeraWhole insect16.780.172,011,619
ArthropodZyginidia pullulaWhole insect15.880.383,745,691
FishSalmo salaraMeat20.690.11240,091
FungiBoletus edulisaStem part15.420.289,270,902
FungiSaccharomyces cerevisiaeaColony17.970.251,584,262
MammalBos primigenius taurusaMeat25.080.3111,466
MammalCapreolus capreolusaMeat22.570.5765,416
MammalEquus ferus caballusaHair22.950.3950,274
MammalHomo sapiensaBlood26.570.934,103
MammalMus musculusaBlood24.090.2322,820
MammalOvis gmelini ariesaMeat22.190.3684,923
MammalSus scrofaaMeat23.661.0530,811
PlantGinkgo bilobaaLeaf19.890.23418,889
PlantLycopersicon esculentumaLeaf15.400.058,605,181
PlantMalus × domesticaaRoot20.731.69233,529
PlantNicotiana occidentalisaLeaf16.950.152,989,473
PlantOlea europaeaaLeaf20.281.18319,704
PlantPinus cembraaLeaf17.680.311,932,346
PlantPrunus armeniacaaLeaf16.250.255,216,449
PlantPyrus communisaLeaf20.720.53235,152
PlantVitis viniferaaLeaf18.040.211,509,260
  1. Amplification results of the 28S rDNA amplicon in 43 different eukaryotic species with threshold cycle values (Cq) and quantification results based on the tenfold standard dilution series of pJet1.2-28S
  2. aTemplate DNA was diluted to 5 ng/µl