Fig. 7From: Development of a universal endogenous qPCR control for eukaryotic DNA samplesLimitations of SYBR-Green assay. Amplification curves of 28S fragment in an undiluted (red), 1:10 (blue) and 1:100 (green) diluted Aphrophora alni sample using a SYBR-Green (a) and TaqMan® (b) assay. Each dilution step was run as a triplicate. The undiluted sample did not show reliable amplification curves when using SYBR-GreenBack to article page