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Fig. 6 | Plant Methods

Fig. 6

From: Development of a universal endogenous qPCR control for eukaryotic DNA samples

Fig. 6

Comparison between SYBR-Green and TaqMan® chemistry. Amplification of the 28S conserved fragment in four-point tenfold sample dilutions of Aphrophora alni, Laodelphax striatellus, Empoasca vitis, Ginkgo biloba, Malus × domestica, Vitis vinifera and cDNA samples of Malus × domestica and Vitis vinifera run as triplicates. Two different amplification chemistries were used, i.e. SYBR-Green and TaqMan®. Error bars represent SEM and significant differences were determined by multiple t-test with Holm-Sidak correction indicated by *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001

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