Fig. 4
From: Development of a universal endogenous qPCR control for eukaryotic DNA samples
Intraassay and interassay variation. Intraassay and interassay variation of five independent TaqMan® qPCR assays. A six-point serial dilution series of pJET1.2-28S was used as the template for amplification. The bars represent the mean Cq-value of three technical replicates ± SEM (error bars, intraassay variation) and the values over the grouped columns show the mean Cq value ± SEM of the five independent runs (interassay variation). Differences were analyzed using Two-way ANOVA with Tukey’s multiple comparison posttest