Table 4 Derivatization methods and their validation parameters for plant hormone

BRs

Rice seeds (50 mg, FW)

In-tip SPE

4-PAMBA

–

–

LC–MS/MS

0.80–5.70 pg mL⁻¹

2.70–19.00 pg mL⁻¹

[119]

BRs

A. thaliana leaves (2 g, FW)

SPE

BPBA

− 20 °C

2 h

UHPLC-ESI-QqQ-MS

2.00–8.00 ng L⁻¹

6.00–23.00 ng L⁻¹

[31]

BRs

A. thaliana and rice (3 g, FW)

SPE

DMAPBA

40 °C

1 h

UPLC-QTOF MS

0.51–1.17 pg g⁻¹

1.70–3.90 pg g⁻¹

[128]

BRs

Rice leaves (5 ± 0.1 mg FW)

PT-SPE

BTBA

80 °C

30 min

UPLC-MS/MS

0.0065–0.021 ng g⁻¹

0.022–0.068 ng g⁻¹

[115]

BRs

Rice (250 mg, FW)

SPE

MPyBA

75 °C

–

UPLC-QTOF–MS

0.34–0.44 pg

1.12–1.45 pg

[129]

BRs

Tomato leaves (225 mg FW)

2DμSPE

m-APBA

–

–

HPLC–MS/MS

0.14–0.66 pg mL⁻¹

0.47–2.20 pg mL⁻¹

[57]

SA, IAA, IPA, IBA, ABA, JA, GA₄

A. thaliana (0.8 mg DW)

SPE

BETA

95 °C

3 h

UPLC–MS/MS

0.61-1.85 pg mL⁻¹

1.98-6.17 pg mL⁻¹

[112]

SA, IAA,ABA, JA, GA_S

Rice leaves (3 g, FW)

SPE

BTA/TEA = (1/1)

105 °C

10 min

CE-ESI-TOF-MS

0.34–4.59 ng mL⁻¹

1.12–15.3 ng mL⁻¹

[91]

GAs

A. thaliana leaves (0.3 mg, FW)

MSPD

BPTAB

85 °C

–

UPLC-MS/MS

0.16–1.31 pg mL⁻¹

0.54–4.37 pg mL⁻¹

[118]