Table 1 Representative sample pretreatment methods for the determination of plant hormone

BRs

80% methanol

Solid phase extraction (SPE)

A. thaliana leaf (10 g FW)

Off-line

[31]

IAA, ABA, JA, GAs

Acetonitrile

Sequential magnetic solid-phase extraction (MSPE)

Brassica napus L. flowers (100 mg FW)

Off-line

[16]

BRs

Methanol

matrix solid-phase dispersion (MSPD)

Rice (200 mg FW)

On-line

[37]

CKs, ABA, GAs, JAs, SA, BRs, SLs

2-proponal/H₂O/HCl (2:1:0.002 v/v/v)

Solid phase extraction (SPE)

P. pinaster Aiton. clones needles (200 ± 20 mg DW)

Off-line

[54]

BRs

Acetonitrile

two-dimensional microscale solid phase extraction (2DμSPE)

Tomato leaves (225 mg FW)

On-line

[57]

SA, IAA, ABA

Methanol

ion-pair stir bar sorptive extraction (IP-SBSE)

Cucumbers and green bean sprouts (100 mg FW)

Off-line

[1]

GAs

75% methanol, 5% formic acid

Liquid–liquid extraction (LLE)

A. thaliana flower

Off-line

[21]

GAs, ABA, ET, SAs, JAs, BRs

Methanol: water: HCl (6 N) (80: 19.9:0.1; v/v/v)

Liquid–liquid extraction (LLE)

Valencia sweet orange (100 ± 2 mg FW)

Off-line

[30]

SA ABA

Methanol–water–acetic acid extractionsolution (80:19:1, v/v/v)

Dispersive liquid–liquid microextraction (DLLME)

Peach (250 mg FW)

Off-line

[40]

JA, IAA, SA, ABA, IBA, GA₃

Centrifugation at 9500 rpm

Single-drop liquid–liquid–liquid microextraction (SD-LLLME)

Fresh fruit juice

Off-line

[58]

ABA, IAA

80% Methanol

Hollow-fiber liquid-phase micro-extraction (HF-LPME)

Soil sample (10 g)

Off-line

[59]

BRs

Acetonitrile

Polymer monolith microextraction (PMME)

Rice shoots (1 g FW leaves, or 0.5 g FW flower tissue)

Off-line

[60]

28-EpihomoBR

80% methanol

Solid-phase microextraction (SPME)

A. thaliana samples (400 mg FW)

On-line

[34]

NAA, 2-NOA, 2,4-D, MCPA, PAA

H₂O,NaCl,HCl

Solid-phase microextraction (SPME)

Tomato (3 g FW)

Off-line

[61]