Table 1 Composition of different media used in the study

Induction medium

1/2 MS, 1 mg/L dichlorophenoxyacetic acid (2,4-d), 3 mg/L 6-benzylaminopurine (6-BA), 30 g/L source (W/V), 8 g/L agar

Proliferation medium

DCR medium, 3% sucrose, 1.13 mg/L 6-BA, 2.21 mg/L 2,4-d and 3 g/L phytagel

Co-cultivation medium

DCR medium, 2.5% sucrose, 1.13 mg/L 6-BA, 2.21 mg/L 2,4-d and 3 g/L phytagel

Suspension medium

10 mM MES, 10 mM Mgcl₂, 0.005% Tween20 and 50, 100, 150, 200 µM AS