Fig. 5
From: An efficient system for Agrobacterium-mediated transient transformation in Pinus tabuliformis
Influence of sonication on transient transformation efficiency in explants of P. tabuliformis. a Effect of sonication on transient transformation using A. tumefaciens LBA4404. Needles were infected for 30 min and sonicated (5, 10, and 15 min). b Effect of sonication on transient transformation using A. tumefaciens GV3101. Needles were sonicated (5, 10, and 15 min) and infected for 30 min. c Effect of sonication on transient transformation using A. tumefaciens LBA4404. Hypocotyls were infected for 30 min and sonicated (5, 10, and 15 min). d Effect of sonication on transient transformation using A. tumefaciens GV3101. Hypocotyls were sonicated (5, 10, and 15 min) and infected for 30 min. z, no GUS expression in needles or hypocotyl fragments; s, GUS expression in single-end needle or hypocotyl cross-sections; d, GUS expression in double-end needle or hypocotyl cross-sections. Each treatment comprised 100 explants and was performed in triplicate. Data are mean ± SD