Fig. 3
From: An efficient system for Agrobacterium-mediated transient transformation in Pinus tabuliformis
Effects of key factors on transient transformation using A. tumefaciens LBA4404 and GV3101. a Effect of Agrobacterium concentration on transient transformation (OD600 values of 0.2, 0.4, 0.6, 0.8, and 1.0). b Effect of infection time on transient transformation (10, 20, 30, and 40 min). c Effect of AS concentration on transient transformation (50, 100, 150, and 200 µM). d Effect of co-cultivation duration on transient transformation (1, 2, 3, 4, and 5 days). Each treatment comprised 27–30 explants and experiments were performed in triplicate. Data are mean ± SD