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Fig. 4 | Plant Methods

Fig. 4

From: Agrobacterium tumefaciens mediated transformation of the aquatic carnivorous plant Utricularia gibba

Fig. 4

Confocal and Nomarski microscopy of U. gibba plants. a Wild type trap stained overnight for GUS activity; b transformed p35S-GUS::GFP trap, with GUS expression visible in antennae, trap hairs and the globose trap body itself; c transformed pRib-GUS::GFP trap, GUS activity is observed in quadrifid cells; d wild type trap, no GFP fluorescence is observed; e, f transformed p35S-GUS::GFP U. gibba showing fluorescence throughout bladder traps; g close up of antennae and trap hairs of transformed U. gibba; h, i transformed pRib-GUS::GFP trap, where fluorescence is observed only in quadrifid cells. Confocal photographs were taken using a LSM810 Zeiss Microscope and Nomarski photographs using a Leica DRM microscope. Scale bars in ah, 50 μM. Bar in i, 20 μM

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