Fig. 2
From: Agrobacterium tumefaciens mediated transformation of the aquatic carnivorous plant Utricularia gibba
Regeneration and GUS staining kinetics of transformed U. gibba plants. a–c Staining pattern of U. gibba tissue transformed with a plasmid carrying p35S-GUS::GFP after 7 days of co-culture with Agrobacterium; d–f GUS staining of Utricularia tissue after 18 days of co-culture; g, h initial transformed buds regenerating after 31 days of co-culture; (i) emerging transformed shoot after 43 days of co-culture; j, l green shoots growing in selective medium after 45 days of co-culture, with dead, whitish tissue observed around them; k, m the same shoots stained for GUS activity are shown. Photographs were taken using a SZH10 Olympus microscope. Scale bar for a–i, l and m represents 0.5 mm; scale bar for j, k represents 1 mm