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Table 4 Transformation efficiency relative to the experiment in which resistant callus or transgenic plants were produced

From: Development of a system for efficient callus production, somatic embryogenesis and gene editing using CRISPR/Cas9 in Saffron (Crocus sativus L.)

Type of explant

Age of the explant (in days)

Inoculated embryo (A)

No. of calli produced in media with cefotaxime

Transgenic plants (B)

Transformation efficiency (B/A  %)

Immature embryo

30

52

2

0

0

Immature embryo

40

50

3

1

2

Immature embryo

35

50

4

0

0

Somatic embryo

30

120

8

3

3

Somatic embryo

35

56

5

2

4

Somatic embryo

40

98

6

3

3