Table 3 Effect of plant growth regulators in combination with MS medium on cormlet formation from in vitro raised shoots
Combination of PGRs used with other supplements | Response | |||||||
|---|---|---|---|---|---|---|---|---|
BAP (mg/L) | NAA (mg/L) | TDZ (mg/L) | IAA (mg/L) | IBA (mg/L) | Sucrose (g/L) | Activated charcoal (g/L) | Paclabutrazole (mg/L) | No. of cormlets formed/flask (n = 5) Mean ± SE |
4.5 | 8.0 | – | – | – | 3.0 | – | – | 1.6 ± 0.0048 |
– | – | 1.0 | – | 0.5 | 3.5 | – | 2.0 | 0.2 ± 0.0032 |
– | – | 0.5 | 1.0 | – | 4.0 | 0.1 | – | 6.8 ± 0.004 |
– | – | 3 | 2 | – | 3.0 | – | – | 2.0 ± 0.008 |
– | – | 1 | 0.5 | – | 6.0 | – | – | 2.2 ± 0.004 |
– | – | 1 | 0.5 | – | 8.0 | – | – | 1.2 ± 0.004 |