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Table 3 Effect of plant growth regulators in combination with MS medium on cormlet formation from in vitro raised shoots

From: Development of a system for efficient callus production, somatic embryogenesis and gene editing using CRISPR/Cas9 in Saffron (Crocus sativus L.)

Combination of PGRs used with other supplementsResponse
BAP (mg/L)NAA (mg/L)TDZ (mg/L)IAA (mg/L)IBA (mg/L)Sucrose (g/L)Activated charcoal (g/L)Paclabutrazole (mg/L)No. of cormlets formed/flask (n = 5) Mean ± SE
4.58.03.01.6 ± 0.0048
1.00.53.52.00.2 ± 0.0032
0.51.04.00.16.8 ± 0.004
323.02.0 ± 0.008
10.56.02.2 ± 0.004
10.58.01.2 ± 0.004
  1. Number of explants inoculated per flask was ten; five replicates of each combination were laid (total number of explants 50/combination). Data was collected after 8 weeks