Skip to main content

Table 2 Effect of plant growth regulators in combination with MS medium on somatic embryogenesis

From: Development of a system for efficient callus production, somatic embryogenesis and gene editing using CRISPR/Cas9 in Saffron (Crocus sativus L.)

Combinations of Plant growth regulators used

Response

BAP (mg/L)

NAA (mg/L)

TDZ (mg/L)

IAA (mg/L)

KIN (mg/L)

Picloram (mg/L)

Activated Charcoal (mg/L)

Number of somatic embryos formed/flask (n = 5) Mean ± SE

–

–

8.8

3.0

1.0

–

–

2.6 ± 0.0048

2.0

0.5

–

–

–

–

–

5.6 ± 0.0048

–

–

1.0

2.6

–

–

–

4.0 ± 0.008

–

–

0.5

–

–

0.5

–

4.4 ± 0.004

–

–

1.0

0.5

–

–

–

5.8 ± 0.012

–

–

0.5

1.0

–

–

0.1

8.4 ± 0.004

  1. Number of explants inoculated per flask was ten; five replicates of each combination were laid (total number of explants 50/combination). Data was collected after 8 weeks. Sucrose concentration in all the media was 3 g/L