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Table 2 Effect of plant growth regulators in combination with MS medium on somatic embryogenesis

From: Development of a system for efficient callus production, somatic embryogenesis and gene editing using CRISPR/Cas9 in Saffron (Crocus sativus L.)

Combinations of Plant growth regulators usedResponse
BAP (mg/L)NAA (mg/L)TDZ (mg/L)IAA (mg/L)KIN (mg/L)Picloram (mg/L)Activated Charcoal (mg/L)Number of somatic embryos formed/flask (n = 5) Mean ± SE
8.83.01.02.6 ± 0.0048
2.00.55.6 ± 0.0048
1.02.64.0 ± 0.008
0.50.54.4 ± 0.004
1.00.55.8 ± 0.012
0.51.00.18.4 ± 0.004
  1. Number of explants inoculated per flask was ten; five replicates of each combination were laid (total number of explants 50/combination). Data was collected after 8 weeks. Sucrose concentration in all the media was 3 g/L