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Table 1 Effect of plant growth regulators in combination with MS medium, on callus induction from corms of saffron

From: Development of a system for efficient callus production, somatic embryogenesis and gene editing using CRISPR/Cas9 in Saffron (Crocus sativus L.)

Combination of Plant growth regulators usedResponse
BAP (mg/L)NAA (mg/L)2,4-D (mg/L)TDZ (mg/L)IAA (mg/L)Number of explants forming calli/petriplate (n = 5) Mean ± SE
1.00.53.6 ± 0.00049
4.04.05.0 ± 0.0081
1.04.0 ± 0.0081
1.00.51.07.6 ± 0.0049
 1.01.03.8 ± 0.011
6.01.94.6 ± 0.049
  1. Number of explants inoculated per petriplate was ten; five replicates of each combination were laid (total number of explants 50/combination). Data was collected after 8 weeks. Sucrose concentration in all the media was 3 g/L