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Table 1 Effect of plant growth regulators in combination with MS medium, on callus induction from corms of saffron

From: Development of a system for efficient callus production, somatic embryogenesis and gene editing using CRISPR/Cas9 in Saffron (Crocus sativus L.)

Combination of Plant growth regulators used

Response

BAP (mg/L)

NAA (mg/L)

2,4-D (mg/L)

TDZ (mg/L)

IAA (mg/L)

Number of explants forming calli/petriplate (n = 5) Mean ± SE

1.0

0.5

–

–

–

3.6 ± 0.00049

–

4.0

–

4.0

–

5.0 ± 0.0081

1.0

–

–

–

–

4.0 ± 0.0081

1.0

–

0.5

–

1.0

7.6 ± 0.0049

 

–

1.0

–

1.0

3.8 ± 0.011

6.0

–

1.9

–

–

4.6 ± 0.049

  1. Number of explants inoculated per petriplate was ten; five replicates of each combination were laid (total number of explants 50/combination). Data was collected after 8 weeks. Sucrose concentration in all the media was 3 g/L