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Fig. 4 | Plant Methods

Fig. 4

From: Development of a system for efficient callus production, somatic embryogenesis and gene editing using CRISPR/Cas9 in Saffron (Crocus sativus L.)

Fig. 4

Southern blot showing the differential DNA copy number of Cas9 gene in various transgenic T0Crocus sativus lines. The figure represents: PC as positive control; WT as wild type and lanes 1–7 demonstrate different transgenic saffron lines. These observations were obtained by the digestion of genomic DNA from seven transgenic plants using restriction enzyme EcoRV and Cutsmart 2 buffer obtained from NEB

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