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Fig. 2 | Plant Methods

Fig. 2

From: Development of a system for efficient callus production, somatic embryogenesis and gene editing using CRISPR/Cas9 in Saffron (Crocus sativus L.)

Fig. 2

Schematic representation of the T-DNA region of the binary vector pYLCRISPR/Cas9 (modified pCAMBIA1300 backbone) developed in-house and utilized for Crocus sativus agro-mediated transformation revealing the 35S promoter:Cas9:NOS terminator cassette; MCS for single or multiple sgRNA insertion; RB: right border; LB: left border

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Plant Methods

ISSN: 1746-4811