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Fig. 1 | Plant Methods

Fig. 1

From: Development of a system for efficient callus production, somatic embryogenesis and gene editing using CRISPR/Cas9 in Saffron (Crocus sativus L.)

Fig. 1

In vitro development of microcorms as a means of cormlet regeneration. The inserts include a corms as explant on first day of inoculation b callus induction on complete MS media supplemented with 2,4-D (0.5 mg/L), BAP (1 mg/L), IAA (1 mg/L) after 2 weeks of culture (c) raised embryogenic calli using complete MS medium supplemented with TDZ (0.5 mg/L) + IAA (1 mg/L) + Activated charcoal (0.1 g/L) after 4 weeks of culture (d) multiplied somatic embryos maintained on using complete MS medium supplemented with TDZ (0.5 mg/L) + IAA (1 mg/L) + Activated charcoal (0.1 g/L) after 2 weeks of culture (e) In vitro shoot initiation after 4 weeks of culture (f) developed cormlets formed from proliferated calli after 8 weeks of culture

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Plant Methods

ISSN: 1746-4811