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Fig. 7 | Plant Methods

Fig. 7

From: A new technical approach for preparing frozen biological samples for electron microscopy

Fig. 7

Components for cooling the HPF-device. a Cooling chamber for cooling the loading area of the HPF-device (top view, lid removed). Walls out of Plexiglas® (1) enclose the entire loading area (2), while a fan (3) is generating airflow to the pan (4) which in normal operation is filled with LN2. As a consequence evaporation of LN2 will be increased and the loading area will rapidly be filled with cold, gaseous N2 which, on the other hand, cools all components and metal surfaces of the loading area, and prevents introduction of water vapour from the ambient atmosphere. For automatic temperature control a thermocouple sensor (5) is installed. The copper strains (6) of the inserted precooling device (PD) (7) lead to the pan and are surrounded by LN2. b PD made from a standard loading device. The internals were removed and replaced by an alloy rod (8) to which a standard specimen pod (9) and a copper strain (10) were screwed

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