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Fig. 3 | Plant Methods

Fig. 3

From: A new technical approach for preparing frozen biological samples for electron microscopy

Fig. 3

TEM micrographs of the alga Micrasterias denticulata (a, b) and the alga Klebsormidium crenulatum (c–g) after freezing (b, d, g) in comparison to 20 °C controls (a, c, e, f). a 20 °C control of Micrasterias shows single mitochondria (m), mucilage vesicles (mv), a part of the chloroplast (chl) and a part of the vacuole (v). bMicrasterias cell after − 2 °C freezing with aggregated and fused mitochondria (m), endoplasmic reticulum (er), dictyosome (d), mucilage vesicles (mv) and chloroplast (chl). c 20 °C control of Klebsormidium shows vacuole (v) and cytoplasm (cyt) with single mitochondrion (m), chloroplast (chl), dictyosome (d), multi vesicular body (mvb), peroxisome (p) and nucleus (n). dKlebsormidium after − 2 °C freezing shows chloroplast (chl), nucleus (n), peroxisome (p), vacuole (v) and cell wall (cw). e High magnification of 20 °C control of Klebsormidium shows double membranes (arrows) and cristae (asterisks) of mitochondrion (m) and chloroplast (chl). f High magnification of 20 °C control of Klebsormidium with chloroplast (chl) and cytoplasm (cyt), surrounded by the plasma membrane with the typical two-leaflet structure (arrows) and cell wall (cw). g High magnification of Klebsormidium frozen at − 2 °C shows peroxisome (p), vacuole (v), cell wall (cw) and double membranes (arrows) of chloroplast (chl) and nucleus (n)

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