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Fig. 1 | Plant Methods

Fig. 1

From: A new technical approach for preparing frozen biological samples for electron microscopy

Fig. 1

Sample temperatures during controlled freezing prior to HPF. Temperature of (1) M. denticulata and (2) K. crenulatum during linear cooling (− 2 °Cˑh−1) and subsequent preparation of the supercooled (unfrozen) algae at − 2 °C followed by inductance of ice formation (not shown). (3) Leaf temperature of R. glacialis. During linear cooling (− 3 °Cˑh−1) a clearly visible freezing exotherm, indicating extracellular freezing of the leaf mesophyll, occurred at -3.2 °C (horizontal arrow). (4) Temperature of Lemna sp. during linear cooling to − 2 °C (dotted). (5) Leaf temperature of P. mugo needles during freezing to − 6 °C (Arrow: freezing exotherm; asterisk: transient temperature deviations caused by technical reasons

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