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Fig. 5 | Plant Methods

Fig. 5

From: A convenient, rapid and efficient method for establishing transgenic lines of Brassica napus

Fig. 5

Convenient screening during seed germination. a–m Observation of red fluorescence through the primary stage of seedling. The red fluorescence could be observed inthe cotyledon (b, f, j), hypocotyl (c, g, k), seed coat (d, h, l) and root (e, i, m) of rapeseed seedlings under a laser confocal fluorescence microscope FV1000, respectively. n RT-PCR identification of the relative gene expression in seedlings. The upper diagram represents the relative gene expression of DsRed in seedlings from different RNAi lines. The middle diagram represents the relative gene expression of BnaA07g17400D in seedlings from different RNAi lines. The lower diagram represents the relative gene expression of DsRed in roots, seed coats, hypocotyls and cotyledons from seedlings of different RNAi lines

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