Fig. 2

Overview of the efficient method for the high-efficiency acquisition of transgenic lines combined with a visual screening method compared with antibiotics screening in B. napus. a Highly efficient genetic transformation combined with a convenient visual screening marker DsRed via Agrobacterium-mediated hypocotyl transformation in B. napus. Hypocotyls were transformed with a vector accompanied by DsRed and hygromycin via Agrobacterium- mediated transformation. The light grey lines inside the oval represent hypocotyls from B. napus. b , c Calli formation and screening during the callus-induced stage with or without the visual screening method. The calli would survive under antibiotics screening with hygromycin in b, and they were further picked by the visual screening using a hand-held green fluorescent flashlight in c. The endpoints in the line represent the formed callus, and the red spots in the endpoints represented the red fluorescence observed in the calli. d ,e Shoots formation in shoot-induced medium with or without the visual screening method. The calli would survive under antibiotics screening with hygromycin in d, and they were further picked for visual screening using a hand-held green fluorescent flashlight in e. The red spots in the shoots represent the red fluorescence observed in the cotyledons. f, g highly efficient screening of the transgenic-positive rooted T0 plantlets and T1 transgenic-positive plants. Transgenic-positive rooted T0 plantlets and T1 transgenic-positive plants could be rapidly and high efficiently obtained when combined with the convenient visual screening method. h Visual screening in cotyledons, hypocotyls, seed coats and roots during seed germination. The seedlings could be screened with the visual marker DsRed using laser confocal fluorescence microscopy