Fig. 1
From: A convenient, rapid and efficient method for establishing transgenic lines of Brassica napus
Vectors construction for gene over-expression and knock-down. a Diagram of the construction of the over-expression vector. The expression of BnaA07g17400D and BnaC05g34170D is driven by the glycinin promoter. The N-terminus of BnaA07g17400D and BnaC05g34170D is accompanied by a flag. b Diagram of the construction of the RNAi knock-down vector. The expression of sense strands and antisense strands is driven by the 2 × 35S promoter and a 2 × nuclear localization signal (NLS) at the N-terminus. DsRed in both vectors was also under the control of the CaMV35S promoter