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Fig. 2 | Plant Methods

Fig. 2

From: An improved growth medium for enhanced inoculum production of the plant growth-promoting fungus Serendipita indica

Fig. 2

S. indica chlamydospore production on agar medium. a Image of fungal growth on CM agar and VJS agar 8 days after inoculation with an agar plug. Standard Petri dishes with a diameter of 9 cm were used and kept at room temperature (20 ± 3 °C) in the dark. b Amount of chlamydospores harvested from agar plates. Chlamydospores were obtained by washing the surface of the plates with 15 ml of distilled water using a spatula, and subsequent filtering through Miracloth to remove hyphae and debris. After appropriate dilution, chlamydospores were counted using a microscope and a Fuchs-Rosenthal counting chamber. Values are means from three plates, with error bars indicating standard error. The values were subjected to a one-way analysis of variance (ANOVA) followed by Tukey’s post-hoc-test. Mean values significantly different at an alpha level of 0.05 are indicated by different letters. Experiments were independently reproduced at least three times with similar results

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