Fig. 1

Serendipita indica biomass production in different media. a The fungus was grown in 100 ml of medium in 300 ml Erlenmeyer flasks for 10 days at room temperature (20 ± 3 °C) on a shaker (70 rpm). Mycelium was retrieved using Miracloth and fresh weight was determined subsequently. Media used: Complex medium ‘CM’; Vegetable juice ‘VJ’ medium: 150 ml V8 juice, 850 ml distilled water and 5 mM CaCO₃; ‘7% VJ’ medium’: VJ medium with 70 ml instead of 150 ml V8 juice; ‘VJ + d-glucose’: VJ medium supplemented with 10 g/L d-glucose; ‘VJ-Ca’ medium: VJ medium without CaCO₃. Values are means of three cultures, with error bars indicating standard deviation. The values were subjected to a one-way analysis of variance (ANOVA) followed by Tukey’s post-hoc-test. Mean values significantly different at an alpha level of 0.05 are indicated by different letters. Experiments were independently reproduced at least three times with similar results. b Diameter of fungal colonies on agar plates prepared with VJ medium or VJS medium. VJS medium is VJ medium supplemented with 40 g/L sucrose and with CaCO₃ to a final concentration of 20 mM. Values are means of three cultures, with error bars indicating standard deviation. A Student’s t-test was calculated, and the asterisk indicates a p-value lower 0.05. Experiments were independently reproduced at least three times with similar results