Simple semi-high throughput determination of activity signatures of key antioxidant enzymes for physiological phenotyping

Table 1 Activity profile of the nine antioxidant enzymatic signature in different plants and tissue

	Arabidopsis leaves	Maize leaves	Maize roots	Sugar beet roots	Barley leaves
SOD	44.2 ± 2.05	15.4 ± 5.50	26.4 ± 5.34	8.02 ± 4.08	21.1 ± 1.05
POX	0.431 ± 0.106	3.71 ± 1.07	N.D.	0.131 ± 0.0220	8.81 ± 0.926
cwPOX	8.18 ± 1.01	1.20 ± 0.156	1.06 ± 0.931	1.27 ± 0.0373	4.73 ± 0.283
CAT	2.17 ± 0.300	0.226 ± 0.0581	0.0389 ± 0.0455	0.217 ± 0.0470	0.761 ± 0.123
APX	N.D.	2.73 ± 1.62	3.23 ± 4.04	N.D.	1.97 ± 0.977
MDHAR	3.02 ± 0.951	N.D.	N.D.	0.464 ± 0.0402	0.302 ± 0.0633
DHAR	5.02 ± 0.485	N.D.	N.D.	0.353 ± 0.103	0.235 ± 0.0238
GR	6.91 ± 0.514	1.45 ± 0.499	0.0199 ± 0.0348	N.D.	0.495 ± 0.203
GST	8.25 ± 0.0597	0.0298 ± 0.0126	0.183 ± 0.0539	4.28 ± 0.122	5.11 ± 0.0935

The values are normalized per fresh weight (Mean v [nkat/g FW]) ± standard deviation
The activities for Arabidopsis leaves, maize leaves and maize roots are average of 3 independent plant replicates whereas for sugar beet roots and barley leaves 3 distinct extractions were performed on the same plant sample, consisting of at least 15 individual plants for barley leaves and 3 plants for sugar beet roots
N.D. not detectable

ISSN: 1746-4811