Fig. 5
From: Protocol: low cost fast and efficient generation of molecular tools for small RNA analysis
a Northern blot analysis of MIM319 (IPS1 probe, upper panels) and miR319 (miR319 probe; lower panels) of N. benthamiana leaves transiently expressing the empty vector, the MIM319 construct, and/or the miR319 as indicated. Endogenous N. benthamiana miR159 can also be detected using the miR319 probe. Sybr (Thermo Scientific, USA) staining images, used to confirm equal loading, are shown below the corresponding Northern blots. Photographs showing whole plants and leaf detail of wild type Col-0 Arabidopsis plants (b and d) and transgenic plants expressing the MIM319 construct (c and e). f Flowers from wild type Col-0 Arabidopsis plants (left) and transgenic plants expressing the MIM319 construct (right). g Semi-quantitative RT-PCR showing accumulation of the MIM319 non-coding RNA (ncRNA) in inflorescences from MIM319 transgenic lines. Actin was used as an internal control. h RT-qPCR showing the relative levels of miR319 and two target genes (TCP2 and TCP4) in inflorescences from wild type (WT) and MIM319 plants. The average and standard error of three technical replicates is represented