Fig. 4
From: Protocol: low cost fast and efficient generation of molecular tools for small RNA analysis
a Representative images showing the result of plating the full volume of each transformation into DH5α as obtained during the cloning process of individual MIMs (MIM156, MIM160, MIM164, MIM319 and MIM390). b In the top panel, a representative image showing the result of plating the full volume of the transformation into DH5α as obtained during the simultaneous cloning of all five MIMs within a single cloning reaction (mix of duplex pairs for MIM156, MIM160, MIM164, MIM319 and MIM390). In the bottom panel, the pie chart shows the distribution of clones obtained for each MIM construct among 24 colonies randomly selected from the plate shown in the top panel, as determined by sequencing. c The upper panel shows the PCR analysis of five colonies obtained from each of the plates from the individual MIM cloning process (a). The lower panel shows the PCR analysis of 15 colonies obtained from the plate corresponding to the simultaneous cloning of all five MIMs (b). All colonies were confirmed to have lost the ccdB cassette. C+ corresponds to the positive control reaction in which the original plasmid containing ccdB was used as a template in the PCR