Skip to main content
Fig. 4 | Plant Methods

Fig. 4

From: An improved protein lipid overlay assay for studying lipid–protein interactions

Fig. 4

Comparison of CPLO and MPLO assays for the interaction between PG and recombinant protein AtROP6. a Interaction between PG and recombinant protein AtROP6 using the CPLO assay. b Interaction between PG and recombinant protein AtROP6 using the MPLO assay. DGDG, MGDG, PG, SQDG, and MG were dissolved in MeOH, DG was dissolved in chloroform, and the other lipids were dissolved in solvent 1 (65:35:8 chloroform:MeOH:H2O). The lipids were spotted a PVDF membrane and the lipid amount spotted was 5 nmol for each lipid. Lipids in each spot are shown on the left and right. AtROP6 was extracted and purified from Escherichia coli with His-epitope tag. PA phosphatidic acid, LPA lysophosphatidic acid, PI phosphatidylinositol, PS phosphatidylserine, PE phosphatidylethanolamine, PC phosphatidylcholine, DGDG digalactosyldiacylglycerol, MGDG monogalactosyldiacylglycerol, PG phosphatidylglycerol, SQDG sulfoquinovosyl diacylglycerol, MG monoglyceride, DG diglyceride. The strips of CPLO and MPLO assays were performed exposure simultaneously using the same ECL reagent and the same settings

Back to article page