Fig. 3
From: An improved protein lipid overlay assay for studying lipid–protein interactions
Comparison of CPLO and MPLO assays for the interaction between C18:1, C18:2, and C18:3 with AHA2 C-terminus. C18:0, C18:1, C18:2, and C18:3 were dissolved in 1:1 dichloromethane:MeOH and spotted onto a PVDF membrane. Solvent alone served as a negative control. The amount spotted was 5 nmol for each lipid, and lipids in each spot are shown at the top. AHA2 C-terminus was extracted and purified from Escherichia coli with His-epitope tag. The upper and lower lanes show detection using CPLO and MPLO assays, respectively. The strips of CPLO and MPLO assays were performed exposure simultaneously using the same ECL reagent and the same settings