Fig. 2
From: An improved protein lipid overlay assay for studying lipid–protein interactions
Comparison of CPLO and MPLO assays for the interaction between PI3P and G302. Assays were carried out between PI3P and its commercially available interacting protein G302 (Echelon). The amount of PI3P spotted onto a PVDF membrane is shown at the top. The upper and lower lanes show the interactions between PI3P and G302 using CPLO and MPLO assays, respectively. The amount of PI3P-binding G302 is shown on the bottom of the signal. PI3P phosphatidylinositol-3-phosphate. The strips of CPLO and MPLO assays were performed exposure simultaneously using the same ECL reagent and the same settings