Molecular karyotypes of loquat (Eriobotrya japonica) aneuploids can be detected by using SSR markers combined with quantitative PCR irrespective of heterozygosity

Table 3 Primer information for 17 SSRs

SSR name	Primer sequences (5′ → 3′)	Origin	LG in loquat	Product size/bp	T (°C)
TsuENH094	F: GAAGAAGCAAAACCCGAAGA R: TTGTTCTCCTCGCCACCTT	Pear	LG1	155	60
MEST028	F: ATTGGCATTGCTTCTCACC R: TGCAACAACAATTCCCTTCA	Apple	LG2	148	60
CH03g12	F: GCGCTGAAAAAGGTCAGTTT R: CAAGGATGCGCATGTATTTG	Apple	LG3	154–200	60
TsuENH044	F: TGGCTAAATACTCTTCTCGAAAACAA R: GTGATTATTATAGATACCAAGCCTCTC	Pear	LG4	127	60
NZmsCN898349	F: GAGTTGGCAGAAAGAAACCA R: CTGGGTGAAGACGAGATGCT	Apple	LG5	200	60
NZmsCO754252	F: CTGCCCTCAAGGAGAATGTC R: ACAGGTGCAGCAAAGGCTAT	Apple	LG6	195	60
NZmsEB137749	F: ATCTCCTGCTGTGCTGGTCT R: TCACCAAACACCAATCAACAA	Apple	LG7	220	60
TsuENH034	F: CATTATCCATTTGATTAAACTACACG R: GGTAGAAAGAGAAGGAAAGTGGG	Pear	LG8	151	60
TsuENH097	F: CTGACACCCACTACGATTCAAGA R: AAACGAGCTTGGTACGGATTACA	Pear	LG9	162	60
Hi05b02	F: GATGCGGTTTGACTTGCTTC R: GTTTCTCCAGCTCCCATAGATTGC	Apple	LG10	120–178	60
IPPN14	F: GAGGAAGTAACCGCATCAGC R: TCTAAGGGCAGGCAGATCAC	Pear	LG11	223–252	60
MEST011	F: GCGTGAGTTGAGCAAGATGG R: TAGAAGCAATAAGGTGGAGTGGT	Apple	LG12	205	60
CH02e02	F: CTCATCAGTCTCACTGACTGTGTG R: AGGGTCAGGGTCAGTCAGG	Apple	LG13	122–130	60
TsuENH093	F: AGACTGCTGAGGGAATCCATAA R: TTCCGAGTCAAATGGGGC	Pear	LG14	144	60
TsuENH007	F: ATTCATTGCACCGACTACCGATT R: AGTGGCGTAGTGGGAAGGG	Pear	LG15	166	60
Hi22f06	F: CAATGGCGTCTGTGTCACTC R: GTTTACGACGGGTAAGGTGATGTC	Apple	LG16	240–246	60
TsuENH002	F: CAGCAGGAAACACAGAAAAACAG R: ATATCGAGCAATCAAGGAAGCAG	Pear	LG17	116	60

The primers for TsuENH094, TsuENH044, TsuENH034, MEST011, TsuENH093, and TsuENH007 were designed by the authors based on the expressed sequences (National Center for Biotechnology Information, NCBI). The information on the LGs in loquat was obtained from Fukuda et al. [27]

ISSN: 1746-4811