Fig. 2

Sample collection with laser micro-dissection for ICP-MS analysis. Schematic illustration of a cross section from barley roots, mounted on a PEN or PET plastic membrane (a). Laser capture microdissection collection of the stele and cortex tissues from a 60 µm thick cross section of barley root (b). The area of the stele is about 30,000 µm² and the area of the cortex is about 150,000 µm², corresponding to volumes of 0.0018 and 0.0090 mm³, or 1.8 and 9 nL, respectively, of the root cylinder