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Fig. 4 | Plant Methods

Fig. 4

From: Development of a DNA-based real-time PCR assay for the quantification of Colletotrichum camelliae growth in tea (Camellia sinensis)

Fig. 4

Quantification of C. camelliae CCA on tea plant cultivars LJ43 and ZC108. a Photos of anthracnose leaf symptoms developed on LJ43 and ZC108 over a time course of 2 days, 4 days and 6 days post C. camelliae CCA inoculation. b The growth of C. camelliae CCA on LJ43 and ZC108 was determined by visual lesion measurement. c, d qRT-PCR based biomass of the C. camelliae CCA growth on tea plants LJ43 and ZC108, respectively. c The ratio of the primer pairs GAPDH/Cs18SrDNA1 was used to determine the fungal biomass. d The ratio of the primer pairs ITS/Cs18SrDNA1 was used to determine the fungal biomass. The letters a and b represent significant differences between different samples according to LSD test (P < 0.05)

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