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Fig. 3 | Plant Methods

Fig. 3

From: Development of a DNA-based real-time PCR assay for the quantification of Colletotrichum camelliae growth in tea (Camellia sinensis)

Fig. 3

Comparative analysis of the two quantification methods for C. camelliae CCA growth over the time course of infection. a Photos of anthracnose leaf symptoms developed from 1 to 6 days after inoculation on tea cultivar LJ43. b The growth of C. camelliae CCA on tea plant was determined by the classical visual lesion measurement method over a time course of 6 days. c, d qRT-PCR-based biomass of C. camelliae CCA growth over 6 days. c The ratio of the primer pairs GAPDH/Cs18SrDNA1 was used to determine the fungal biomass. d The ratio of the primer pairs ITS/Cs18SrDNA1 was used to determine the fungal biomass. The letters represent significant differences at different times by LSD test (P < 0.05)

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