Fig. 3From: Development of a DNA-based real-time PCR assay for the quantification of Colletotrichum camelliae growth in tea (Camellia sinensis)Comparative analysis of the two quantification methods for C. camelliae CCA growth over the time course of infection. a Photos of anthracnose leaf symptoms developed from 1 to 6 days after inoculation on tea cultivar LJ43. b The growth of C. camelliae CCA on tea plant was determined by the classical visual lesion measurement method over a time course of 6 days. c, d qRT-PCR-based biomass of C. camelliae CCA growth over 6 days. c The ratio of the primer pairs GAPDH/Cs18SrDNA1 was used to determine the fungal biomass. d The ratio of the primer pairs ITS/Cs18SrDNA1 was used to determine the fungal biomass. The letters represent significant differences at different times by LSD test (P < 0.05)Back to article page