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Fig. 2 | Plant Methods

Fig. 2

From: Development of a DNA-based real-time PCR assay for the quantification of Colletotrichum camelliae growth in tea (Camellia sinensis)

Fig. 2

qRT-PCR methodology to quantify C. camelliae growth rate following infection of tea plant leaves. a Photo of tea plant cultivar LJ43 infested with C. camelliae in a tea garden. The close-up frame shows a single leaf with typical anthracnose symptoms. b Phenotypes of LJ43 leaves in response to C. camelliae CCA (Cc, top row) and ddH2O control (CK, bottom row). The leaves were wounded with a razor blade and immediately inoculated with 5 µL C. camelliae CCA spores (1 * 106 spores mL−1). For the control, ddH2O alone was used. c, d qPCR-based biomass of C. camelliae CCA growth on tea plant LJ43. c The ratio of the primer pairs GAPDH/Cs18SrDNA1 was used to determine the fungal biomass. d The ratio of the primer pairs ITS/Cs18SrDNA1 was used to determine the fungal biomass. *P < 0.05 by the LSD test

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Plant Methods

ISSN: 1746-4811