Fig. 1

Leaves damaged by rice blast fungus were dyed. Leaves were either treated with water (NS, no staining) or dyed with red or blue cut-flower dye at 1 to 3 days post-inoculation (DPI). The lower (abaxial) side of the leaf blades is shown. Yellow arrows indicate target lesions. Yellow brackets mark areas that retain the original leaf tissue colors. Upper images in each pair show light microscopy results; lower images show fluorescence microscopy results. Spores and appressoria appeared on the upper epidermis. White arrowheads and white lines indicate hyphae detected with fluorescent wheat germ agglutinin conjugate. White bars, 2000 µm; black bars, 100 µm