Table 1 Overview of the phenotyping methodology and trait derived from the corresponding methods in the study. R, Red; G, Green; B, Blue
Phenotyping | Analysis method | Traits extracted | Description |
|---|---|---|---|
Developing panicle (week 1, 2 and 3 post-fertilization) | Reconstruction of 3D point cloud from multi-view images | Voxel count | Total number of points in 3D reconstructed point cloud, which can be used to estimate the overall volume |
Color sum—R, G, B | Sum of color intensities of signals from R, G, and B channels | ||
Color intensity − ratio of R to G | Ratio of intensity in red channel and the intensity in green channel | ||
Multi-view 2D image analysis | Pixel count | Total pixel counts to estimate 2D surface area of the panicle | |
Mature panicle | Single-view conventional 2D scanning | Projected seed count | Estimation of total number of seeds |
Projected surface area | Estimation of total surface area | ||
Seed area | Mean area of all seeds | ||
Seed major and minor axis length | Mean major and minor axis length of all seeds | ||
Manual measurement | Yield-related traits | Total number of seeds, total seed weight, fertility and weight per seed |