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Fig. 3 | Plant Methods

Fig. 3

From: Virus-induced gene silencing (VIGS) in Cannabis sativa L.

Fig. 3

Silencing of PDS induced with the Cotton leaf crumple virus a Plants were infiltrated with the A. tumefaciens strain AGL1 carrying pCotton-PDS or an empty-vector control. Phenotypes could be identified approximately four weeks after infiltration with pCotton-PDS (right), whereas control plants showed no phenotype (left). b Overview on the efficiency of VIGS with different A. tumefaciens strains and number of infiltrated plants. c Quantification of the expression level of PDS in plants showing a phenotype and empty- vector control plants were analyzed by qPCR and normalized relative to the expression levels of eIFa and UBQ5. Error bars indicate standard error of five biological replicates. d Quantification of pigment content (chlorophyll a, chlorophyll b and carotenoids) of leaves from empty-vector control plants and PDS-silenced plants. Error bars indicate standard error of 3 representative replicates

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