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Fig. 2 | Plant Methods

Fig. 2

From: Virus-induced gene silencing (VIGS) in Cannabis sativa L.

Fig. 2

Workflow of a VIGS experiment a after identification of gene sequences and validation by sequencing, suitable siRNAs and off-targets are predicted. Based on these predictions silencing gene fragments were defined. b For in vivo validation silencing fragments are cloned in the VIGS-vectors and transformed in Agrobacteria for infiltration experiments

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Plant Methods

ISSN: 1746-4811