Fig. 3From: A protocol for laser microdissection (LMD) followed by transcriptome analysis of plant reproductive tissue in phylogenetically distant angiospermsCorrelations of RNA quality and quantity, tissue size, sample replicates of RNA of different species (orange: E. californica, blue: A. thaliana, grey: O. sativa, red: excluded samples) and developmental stages (symbols in figures A and B, stage 1: circle; stage 2: triangle; stage 3: square; stage 4: diamond). a RNA quality (RIN) in relation to yield [ng]. b relation of area of tissue used for extraction [mm2] to the RNA quantity [ng]. c Principal component analyses of the A. thaliana RNA Seq samples. S1—S4 show the different developmental stages, a–d corresponds to the four replicas per stage. Node shapes show total RNA (ng): low = 4–15 ng; middle = 16–30 ng; high = 31–45 ng; very high ≥ 46 ng. Different colors correspond to the total area of the collected samples (mm2): XS = 50–100 µm2; S = 101—300 µm2; M = 301—1000 µm2; L = 1001—2000 µm2; XL = 2001—3000 µm2Back to article page