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Table 1 Summary of treatments, materials and atmospheric conditions used for the different experiments

From: Non-invasive diagnosis of viability in seeds and lichens by infrared thermography under controlled environmental conditions

ExperimentDescriptionType of materialAtmosphereSample specification
Exp. 1Effects of relative humidity on the thermal fingerprints of Pisum sativum seedsSeed (P. sativum)Normoxia, 30% RH"Live": germinated (n = 20)
“Dead”: non-germinated (n = 10)
Normoxia, 100% RH"Live": germinated (n = 20)
“Dead”: non-germinated (n = 10)
Exp. 2Effects of imbibition with water vapour and liquid water, and oxygen, on the thermal fingerprints of L. pulmonaria thallus discsLichen (L. pulmonaria)Normoxia, 100% RH"Live": untreated (n = 15)
"Dead": microwaved (n = 15)
Anoxia, 100% RH"Live": untreated (n = 15)
"Dead": microwaved (n = 15)
Exp. 3Thermal fingerprints of the fungal tissue in the lower cortex of L. pulmonaria discsLichen (L. pulmonaria) “upside down”Normoxia, 100% RH"Live": untreated (n = 14)
"Dead": microwaved (n = 14)
Exp. 4Effects of controlled deterioration on the thermal fingerprints of L. pulmonaria, P. furfuracea and P. leucophlebia thallus discs upon imbibitionLichens (L. pulmonaria, P. furfuracea and P. leucophlebia)Normoxia, 100% RH"Live": untreated (n = 14)
"Stressed": 0.3 < Fv/Fm < 0.4 after CD (n = 14)
"Dead": Fv/Fm ≤ 0.2 after CD (n = 14)
Exp. 5Thermal fingerprints of low- and high-molecular-weight carbohydrates upon hydrationCarbohydrate standardsNormoxia, 100% RHStarch (n = 8)
Glucose (n = 8)
Mannitol (n = 8)
Ribitol (n = 8)
  1. Numbers of replicates are shown in brackets