|0.15| °C in defined time windows, and that RH affected the shape of these thermal fingerprints. We demonstrated that IRT can also be used to assess the viability of the lichens Lobaria pulmonaria, Pseudevernia furfuracea and Peltigera leucophlebia. No clear relationship between aerobic metabolism and the shape of thermal fingerprints was found. Conclusions Infrared thermography appears to be a promising method for the diagnosis of viability of desiccation-tolerant tissues at early stages of water uptake. For seeds, it is possible to diagnose viability within the first hours of rehydration, after which time they can still be re-dried and stored until further use. We envisage our work as a baseline study for the use of IR imaging techniques to investigate physiological heterogeneity of desiccation tolerant life forms such as lichens, which can be used for biomonitoring, and for sorting live and dead seeds, which is potentially useful for the seed trade."/>
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Fig. 5 | Plant Methods

Fig. 5

From: Non-invasive diagnosis of viability in seeds and lichens by infrared thermography under controlled environmental conditions

Fig. 5

Effects of controlled deterioration on the thermal fingerprints of L. pulmonaria, P. furfuracea and P. leucophlebia thallus discs upon imbibition (Experiment 4). "Live", "stressed" and "dead" thallus discs are shown in green, orange and red colour, respectively. ac Maximal photochemical efficiency (Fv/Fm) of thallus discs. Bars labelled with different letters indicate significant differences between "live", "stressed" and "dead" thallus discs with high, medium or low Fv/Fm, respectively, at P < 0.05 (one-way ANOVA). Bars are means ± SE of n = 8 thallus discs each. df Moisture contents (MC) of another set of thallus discs during imbibition. Symbols are means ± SE (n = 4). At each time interval, letters indicate significant differences between "live", "stressed" and "dead" thallus discs at P < 0.05 (one-way ANOVA). In d, the green and red lines overlap. gi Thermal fingerprints of "live", "stressed" and "dead" thallus discs, showing median values of relative temperature (rT) during imbibition. Data are medians of n = 14 thallus discs. jl Differences between the fingerprints of "live" vs "dead", and of "stressed" vs "dead" thalli (ΔT). Horizontal green (or orange) bars at the bottom of the graphs indicate the time windows in which the rT of "live" (or "stressed") discs differed significantly from "dead" discs at P < 0.05 (Two Sample t-test). Open blue horizontal bars indicate the time periods of hydration by water vapour and closed blue bars indicate imbibition in liquid water from below

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