Fig. 4

Subcellular localization analysis of ZmbHLH20 and ZmbHLH76 in maize protoplasts. Maize protoplasts were transfected with eGFP (a), OsGhd7 nucleus-targeting sequence fused with eGFP (b), ZmbHLH20-eGFP (c), or ZmbHLH76-eGFP (d). Left to right: eGFP fluorescence channel, chloroplast fluorescence channel, bright-field channel, and merge. Bars = 5 μm