Fig. 4From: An efficient system composed of maize protoplast transfection and HPLC–MS for studying the biosynthesis and regulation of maize benzoxazinoidsSubcellular localization analysis of ZmbHLH20 and ZmbHLH76 in maize protoplasts. Maize protoplasts were transfected with eGFP (a), OsGhd7 nucleus-targeting sequence fused with eGFP (b), ZmbHLH20-eGFP (c), or ZmbHLH76-eGFP (d). Left to right: eGFP fluorescence channel, chloroplast fluorescence channel, bright-field channel, and merge. Bars = 5 μmBack to article page