Fig. 3From: An efficient system composed of maize protoplast transfection and HPLC–MS for studying the biosynthesis and regulation of maize benzoxazinoidsChanges of benzoxazinoid contents induced by overexpressing and silencing ZmBX1 in maize protoplasts. Maize protoplasts were transfected with pM999-eGFP as the control, and simultaneously, maize protoplasts were transfected with pM999-ZmBX1 or pM999-ami-ZmBX1 to overexpress or silence ZmBX1 (OE-ZmBX1 and ami-ZmBX1, respectively). a Relative benzoxazinoid abundances in control and OE-ZmBX1 maize protoplasts. b The accumulation patterns of two main benzoxazinoids, DIMBOA and DIMBOA-Glc in control and OE-ZmBX1 maize protoplasts at different times after transfection. c Relative contents of benzoxazinoids in control and ami-ZmBX1 protoplasts. d The relative expression levels of ZmIGL transcript in OE-ZmBX1 and ami-ZmBX1 protoplasts. Data are means ± SE. Asterisks indicate significant differences between control and overexpression or gene-silenced protoplasts (Student’s t-test; n = 5; *P ≤ 0.05)Back to article page