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Fig. 3 | Plant Methods

Fig. 3

From: An efficient system composed of maize protoplast transfection and HPLC–MS for studying the biosynthesis and regulation of maize benzoxazinoids

Fig. 3

Changes of benzoxazinoid contents induced by overexpressing and silencing ZmBX1 in maize protoplasts. Maize protoplasts were transfected with pM999-eGFP as the control, and simultaneously, maize protoplasts were transfected with pM999-ZmBX1 or pM999-ami-ZmBX1 to overexpress or silence ZmBX1 (OE-ZmBX1 and ami-ZmBX1, respectively). a Relative benzoxazinoid abundances in control and OE-ZmBX1 maize protoplasts. b The accumulation patterns of two main benzoxazinoids, DIMBOA and DIMBOA-Glc in control and OE-ZmBX1 maize protoplasts at different times after transfection. c Relative contents of benzoxazinoids in control and ami-ZmBX1 protoplasts. d The relative expression levels of ZmIGL transcript in OE-ZmBX1 and ami-ZmBX1 protoplasts. Data are means ± SE. Asterisks indicate significant differences between control and overexpression or gene-silenced protoplasts (Student’s t-test; n = 5; *P ≤ 0.05)

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