Fig. 2From: An efficient system composed of maize protoplast transfection and HPLC–MS for studying the biosynthesis and regulation of maize benzoxazinoidsIsolation and transfection of protoplasts from etiolated maize leaves. a Etiolated 12-day-old maize leaves. b The middle leaf segments of etiolated maize seedlings. c Leaf strips. d Leaf strips in protoplast isolation solution. e Removal of leaf strip debris by filtering through a 70-μm cell strainer. f Washing and collecting the maize protoplasts. g, h Bright-field (g) and fluorescence images (h) of the same maize protoplasts expressing eGFP 12 h after transfection. Scale bars = 200 μmBack to article page