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Fig. 4 | Plant Methods

Fig. 4

From: Nourseothricin N-acetyl transferase (NAT), a new selectable marker for nuclear gene expression in Chlamydomonas

Fig. 4

Expression of NAT confers cell resistance to NTC. a A diagram showing the construct for expressing NAT in C. reinhardtii. b WT cells were transformed with plasmid pHR-NAT-HA and grown on TAP agar plates supplemented with or without NTC (10 µg/ml). c Expression of NAT-HA was examined by immunoblotting with anti-HA antibody. α-tubulin was used as a loading control. d The extent of NTC resistance is correlated with the levels of NAT expression. Cells of wild type and strains with higher (#1 and #6) or lower (#2) expression levels of NAT were grown on agar plates supplemented with different concentrations of NTC as indicated

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